mouse anti-gapdh gapdh-71.1, ab_1078991  (Millipore)

Journal: Epigenomes

Article Title: Opposite and Differently Altered Postmortem Changes in H3 and H3K9me3 Patterns in the Rat Frontal Cortex and Hippocampus

doi: 10.3390/epigenomes8010011

Figure Lengend Snippet: Antibodies used in Western blot analysis and confocal immunohistochemistry *.

Article Snippet: GAPDH , Mouse anti-GAPDH, monocl. ab., clone GAPDH-71.1 (G8795) , 1:20,000 , Sigma, St. Louis, MO, USA , Anti-mouse IgG, peroxidase conjug. (WB) (A9044) , Sigma, St. Louis, MO, USA , 1:2000 (WB).

Techniques: Western Blot, Immunohistochemistry

Journal: PLoS ONE

Article Title: A Truncation Variant of the Cation Channel P2RX5 Is Upregulated during T Cell Activation

doi: 10.1371/journal.pone.0104692

Figure Lengend Snippet: A, Western blot of non-activated (CD4 + /−) and activated (CD4 + /+) CD4 + T cell lysates and of P2RX5 cDNA transfected (HEK293/+) or mock-transfected (HEK293/m) HEK293 cell lysates. Blots were stained with anti-P2RX5 antibody. Staining with anti-GAPDH antibody (arrow) served as loading control. B, Western blot of protein biotinylated at the cell surface of activated CD4 + T cells (+) and of controls (−). Protein was fractionated with streptavidin-agarose. B – streptavidin-agarose bound fraction; UB – unbound protein fraction. Western blots were stained with antibodies against P2RX5, GAPDH, and CD3ε as indicated by arrows.

Article Snippet: Primary antibodies were anti-P2RX5 (clone 1C5 for immunoblotting or polyclonal for immunocytochemistry, Abnova, Taipei, Taiwan), anti-talin (clone 8d4, Sigma-Aldrich), anti-GAPDH (clone GAPDH-71.1, Sigma-Aldrich) monoclonal mouse antibodies, and anti-LFA-1 (against integrin, alpha-L, clone EP1285Y, Abcam, Cambridge, UK) monoclonal rabbit antibody.

Techniques: Western Blot, Transfection, Staining

Journal: PLoS ONE

Article Title: A Truncation Variant of the Cation Channel P2RX5 Is Upregulated during T Cell Activation

doi: 10.1371/journal.pone.0104692

Figure Lengend Snippet: A, Western blot analysis of P2RX5, talin, and LFA-1 expression in activated CD4 + T cells transfected with siRNA as indicated. GAPDH served as loading control. B, C, CD4 + T cells were transfected with indicated siRNA followed by activation for 4 or 24 h. Cells were stained with anti-talin antibodies. Scale bar – 10 µm. D, Bar diagram illustrating relative number of polarized CD4 + T cells seen with talin or LFA-1 immunostains. E, Bar diagram illustrating interleukin production of activated non-transfected (grey bars), control siRNA (white bars) or P2RX5-siRNA transfected (black bars) CD4 + T cells (n = 3 donors). Error bars are SEM, n.s. – non significant; * - p<0.05, ** p<0.01 (Student's T-test).

Article Snippet: Primary antibodies were anti-P2RX5 (clone 1C5 for immunoblotting or polyclonal for immunocytochemistry, Abnova, Taipei, Taiwan), anti-talin (clone 8d4, Sigma-Aldrich), anti-GAPDH (clone GAPDH-71.1, Sigma-Aldrich) monoclonal mouse antibodies, and anti-LFA-1 (against integrin, alpha-L, clone EP1285Y, Abcam, Cambridge, UK) monoclonal rabbit antibody.

Techniques: Western Blot, Expressing, Transfection, Activation Assay, Staining